Great work from the Boston Open Source Science Lab (bosslab),
pVIB transformants from BIOTECH 101. 30 second exposure, iso 100, minimum aperture (aperture as big as it can be) with a brief flash of light at the beginning. This develops the scene around the plate, and then the rest of the blue glowing color develops from the long exposure.
The pVIB plasmid contains a luciferase gene (information here) which are enzymes that emit light. This is very cool, great job.
Also related: How Bio-luminescence Works [How Stuff Works]